Table 3.
Primer sequences in RT-qPCR
| Primer | Sequence |
|---|---|
| SNHG1 | F: 5'-GTGACTTCGCCTGTGATGGA-3’ |
| R: 5'-GGCCTCTATCTGTACCTTTATTC-3’ | |
| BMP-2 | F: 5'-AGCCATTGCACACCTCAC-3’ |
| R: 5'-CGTGGTACCAAGAGGACAGAGT-3’ | |
| OCN | F: 5'-TGAGGACCATCTTCTGCTCA-3’ |
| R: 5'-TGGACATGAAGGCTTTGTCA-3’ | |
| RUNX2 | F: 5'-UUGCCCUCUCAACCCAGCUUUU-3 |
| R: 5'-TGGACATGAAGGCTTTGTCA-3’ | |
| miR-320b | F: 5'-CCTGGGAGTATGTCGATCTATTG-3’ |
| R: 5'-TGGTGTCGTGGAGTCG-3’ | |
| IFNGR1 | F: 5'-AAGTGGCGTGTTCAAGTGTG-3’ |
| R: 5'-GGCTTTGGAGATCTGAGCTG-3’ | |
| U6 | F: 5'-CTCGCTTCGGCAGCACA-3’ |
| R: 5'-AACGCTTCACGAATTTGCGT-3’ | |
| GAPDH | F: 5'-CGGACCAATACGACCAA-3’ |
| R: 5'-AGCCACATCGCTCAGACACC-3’ |
Note: RT-qPCR, reverse transcription quantitative polymerase chain reaction; SNHG1, small nucleolar RNA host gene 1; BMP-2, bone morphogenetic protein-2; OCN, osteocalcin; RUNX2, runt-related transcription factor 2; miR, microRNA; IFNGR1, interferon gamma receptor 1; GAPDH, glyceraldehyde-3-phosphate dehydrogenase.