Table 4.
Antibodies used in western blot analysis and immunohistochemistry
| Antibody | Item. No. | Dilution ratio |
|---|---|---|
| BMP-2 | ab214821 | 1: 2000 |
| RUNX2 | ab192256 | 1: 5000 |
| OCN | ab13420 | 1: 2000 |
| JAK2 | ab108596 | 1: 100 |
| P-JAK2 | ab32101 | 1: 100 |
| STAT-1 | ab92506 | 1: 1000 |
| P-STAT-1 | ab109461 | 1: 5000 |
| STAT-2 | ab231184 | 1: 1000 |
| p-STAT-2 | ab53132 | 1: 5000 |
| β-actin | ab179467 | 1: 5000 |
| Secondary antibody | ab150117 | 1: 5000 |
Note: BMP-2, bone morphogenetic protein-2; RUNX2, runt-related transcription factor 2; OCN, osteocalcin; JAK, janus kinase; STAT, signal transducers and activators of transcription.