Table 1.
Target gene | Primers | Product size | |
---|---|---|---|
Occludin | Control | Forward: AGTCCTACCCAGCTACCAAGT | 270bp |
Reverse: TCCTTCTTGGCAACTCACAGT | |||
ZNF765 binding |
Forward: TGGATGGCAACTAACACCTACA | 211bp | |
Reverse: ACAGGACACTTTTCTGCTTCCA | |||
ZO-1 | Control | Forward: GTGCTGGGATTATAAACGTGAGC | 204bp |
Reverse: TGCCTGTAGTCCCAGCTACT | |||
ZNF765 binding | Forward: GCACTTCGAAGCCTTAATTGAT | 207bp | |
Reverse: TGCCAGAAATCCTTAGTAACATCA | |||
Claudin-5 | Control | Forward: GCAGAAGAGGCCAAGGTAAGA | 201bp |
Reverse: ACCTAAGACCCTCCATGGCT | |||
ZNF765 binding | Forward: GCACACTTGCCTTCAGAACC | 205bp | |
Reverse: CTGGTCTGTGCTCAGTTCCA | |||
IGF2BP2 | Control | Forward: TCCTAGGCGTCCCGACTC | 239bp |
Reverse: CAAGGTGGACCGGATGTGAG | |||
ZNF765 binding | Forward: CGCCCGTACAAACTCTCACA | 105bp | |
Reverse: CTCTGCCTCCCTCTCTCTCC |