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. 2020 Nov 26;2020:4830418. doi: 10.1155/2020/4830418

Figure 5.

Figure 5

Nuclear factor E2-related factor 2 (Nrf2) regulates peroxisome proliferator-activated receptor-γ coactivator 1α (PGC1α) at the transcriptional level in SKOV3 cells. (a) Schematic diagram of the sequence spanning from −2000 to +1 relative to the translational start site (TSS) of the PGC1α promoter containing the Nrf2 motif. (b) SKOV3 cells were transfected with Nrf2 or pcDNA3.1 plasmids (vector plasmid as the NC group). Relative NFE2l2 mRNA expression was measured by RT-qPCR. Data are presented as the mean ± SD, n = 3, ∗∗P < 0.01 compared with NC group. (c) The luciferase activities of Epox-treated or Nrf2 overexpression SKOV3 cells transfected with pGL4-PGC1α and Renilla luciferase reporter (pRL-TK) plasmids were determined using dual-luciferase reporter assays. Data are presented as the mean ± SD, n = 3, ∗P < 0.05.