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. 2020 Nov 26;2020:4830418. doi: 10.1155/2020/4830418

Figure 6.

Figure 6

Nuclear factor E2-related factor 2 (Nrf2) activation enhances mitochondrial function in SKOV3 cells. SKOV3 cells were treated with 100 nM Epox for 12 h or transfected with Nrf2 or pcDNA3.1 plasmids (vector plasmid as the NC group). (a) JC-1 staining was used to evaluate MMP, and (b) MitoTracker™ Red staining was used to evaluate the alteration of mitochondrial mass via flow cytometry. (c) ATP production was determined using an ATP Bioluminescence Assay Kit, and (d) relative mtDNA copy numbers were determined by RT-qPCR. Data are presented as the mean ± SD, n = 3, ∗P < 0.05. (e) The oxygen consumption rate was detected using fluorescent oxygen-sensitive probes. Data are presented as the mean ± SD, n = 3, ∗P < 0.05. (f) Mitochondrial proteins were collected, and the expression of mitochondrial respiratory chain proteins was analyzed via Western blotting. Data are presented as the mean ± SD, n = 3, ∗P < 0.05, ∗∗P < 0.01 compared with the control, #P < 0.05, ##P < 0.01 compared with NC.