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Effects of IL-18 on the proliferation of human colon cancer cells. RKO colon cancer cells were transfected with empty vector or plasmids coding for IL-18. The transfection efficiency was assessed by real-time PCR (a) and western blot (b). The effects of IL-18 overexpression on cell proliferation were evaluated with CCK-8 assays (c) and plate colony formation assays (d) (∗P < 0.05).
