(A) HIV-1 Env constructs of three selected RnS-DS-SOSIP constructs highlighted on a phylogenetic tree of the 180 HIV-1 strains.
(B) All three RnS-DS-SOSIP-stabilized constructs yielded binding to a panel of diverse broadly neutralizing HIV-1 antibodies, including prefusion-closed conformation specific antibodies PGT145 or CAP256-VRC26.25, as measured using Octet.
(C) SEC curve and negative-stain EM results for clade AE C1080.c3 RnS-DS-SOSIP-stabilized construct.
(D) SEC curve and negative-stain EM results for clade B REJO.67 RnS-DS-SOSIP-stabilized construct.
(E) SEC curve and negative-stain EM results for clade C 6838.V1.C35 RnS-DS-SOSIP-stabilized construct. Scale bars: 50 nm (representative EM micrographs) and 10 nm (two-dimensional [2D] class average images).
See also Figure S4 and Data S1.