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. Author manuscript; available in PMC: 2020 Dec 3.
Published in final edited form as: Cell Rep. 2020 Nov 24;33(8):108432. doi: 10.1016/j.celrep.2020.108432

Figure 3. Antigenic Analysis and Negative-Stain EM Confirmed Prefusion-Closed Conformation for Selected RnS-DS-SOSIP-Stabilized Constructs.

Figure 3.

(A) HIV-1 Env constructs of three selected RnS-DS-SOSIP constructs highlighted on a phylogenetic tree of the 180 HIV-1 strains.

(B) All three RnS-DS-SOSIP-stabilized constructs yielded binding to a panel of diverse broadly neutralizing HIV-1 antibodies, including prefusion-closed conformation specific antibodies PGT145 or CAP256-VRC26.25, as measured using Octet.

(C) SEC curve and negative-stain EM results for clade AE C1080.c3 RnS-DS-SOSIP-stabilized construct.

(D) SEC curve and negative-stain EM results for clade B REJO.67 RnS-DS-SOSIP-stabilized construct.

(E) SEC curve and negative-stain EM results for clade C 6838.V1.C35 RnS-DS-SOSIP-stabilized construct. Scale bars: 50 nm (representative EM micrographs) and 10 nm (two-dimensional [2D] class average images).

See also Figure S4 and Data S1.