Fig. 3.

Domain organization and amino acid conservation in Ccc1/VIT1 family proteins. Schematic representation of the architecture and key amino acids of the consensus sequence of each group. EgVIT1 group V key amino acids are represented by its IUPAC letter and by the following color code: green for amino acids involved in iron transport from the MBD to the hydrophilic cavity; dark blue, amino acids involved in the transport throughout the cavity; purple, residues implicated in the induction of the seal opening; and red, residues within the MBD. White letters are used when other amino acids are conserved for more than two non-group V protein groups, and circles represent conserved amino acids colored as above for non-group V proteins. Dark and light blue rectangles represent the five TMDs and the three helices, respectively. Orange, grey, green and yellow rectangles symbolize Ferritin-like/erythrin domain, short basic (sB), proline/serine-rich (P/S-rich) and cysteine-rich (C-rich) motives, respectively. Group VII sequences contain a unique short amino acid sequence in the MBD, represented by a dashed line. Numbers indicate the amino acid position in EgVIT1 protein. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)