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. 2020 Aug 3;27(9):836–845. doi: 10.1038/s41594-020-0466-9

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© The Author(s), under exclusive licence to Springer Nature America, Inc. 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 3 — a, Immunofluorescence imaging performed on IMR90 fibroblasts and HEK293T cells infected with either V5-SS18-SSX or V5-SS18. Visualized in red for H3K9me3, SMARCA4, PBRM1, SMARCC1, H3K9Ac across experiments. DAPI is shown as nuclear stain and merged images are provided with scale bars, Scale bar, 5μm. b, IF-based localization of SSX1 FL (1-188aa) in fibroblasts. H2AK119Ub, DAPI counterstain, and merged images are shown. Scale bar, 5μm. c, Peptide competition experiment using Biotinylated SSX peptide (aa 155-188) and unlabeled SSX (aa 155-188). Visualization for Histone H3 using immunoblot. d, Peptide competition experiment using Biotinylated SSX peptide (aa 155-188) and Scrambled control SSX peptide (aa 155-188). Visualization for Histone H3 using immunoblot. e, SSX peptide hybridization experiments performed on methanol-fixed cells. Streptavidin (SA) used for visualization of biotinylated SSX peptides, H2AK119Ub for Barr bodies. DAPI counterstain and merged images shown. Scale bar, 5μm. f, Top, conservation analysis among SSX and PRDM7 and PRDM9 human protein regions. Bottom, peptide pull-down experiments with recombinant nucleosomes performed with Scrambled control SSX1, SSX1, PRDM7, PRDM9. Visualization by colloidal blue staining. g, Left, alignment of SSX proteins (SSX1-9). Right, peptide pull-down experiments with recombinant nucleosomes performed with aa 155-188 of SSX family members. Visualization by colloidal blue staining. Uncropped gel images are presented in Supplementary Figure 1.