Figure 3.

A high level of CXCL-8 resulted from Z-AAT accumulation. MDMs were incubated with LPS (10 ng/ml) overnight. (A) CXCL-8 protein level was measured and compared between LPS-treated M- and Z-MDMs (n=6). (B) Two different concentrations of AAT were extracellularly added to M- and Z-MDM cultures and the cells were incubated with LPS and AAT overnight. Two-way ANOVA test was used to analyze the effect of genotype and treatment (AAT) on the expression of CXCL8 in the cells (n=5). P-values of interaction, genotype, and treatment were 0.96, 0.0001, and 0.996, respectively. *Denotes statistical significance (p-value < 0.05) according to two-tailed Student’s t-test. (C, D) Total proteins were collected from control and LPS-treated MDMs and the increased intracellular AAT levels in LPS-treated cells were visualized and quantified using western blotting (n=5). One-way ANOVA was used to compare the level of intracellular among the samples, and p-value was less than 0.0001. **Denotes statistical significance (p-value < 0.01) according to one-way ANOVA multiple comparisons.