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. 2020 Nov 20;11:574410. doi: 10.3389/fimmu.2020.574410

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Copyright © 2020 Lee, Lu, Oshins, West, Moneypenny, Han and Brantly

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The effect of AAT on LPS-induced neutrophil chemotaxis. MDMs were incubated with LPS (10 ng/ml) overnight. To examine the ability of AAT to regulate neutrophil transmigration, conditioned media of M- and Z-MDMs were collected and placed in the bottom chamber of transwell system. Freshly isolated neutrophils were placed in the top chamber of the transwell system. The number of neutrophils migrated to the bottom layer were counted after 30 min. (A) The number of neutrophils in the bottom layer was divided by the total number of neutrophils to calculate the migration rate of control MDMs. (B) To calculate the migration rate after LPS treatment, the migration rate of control MDM was set to 100%, and the migration rate of LPS-treated MDM was normalized to control for each individual; n=5. *Denotes statistical significance (p-value < 0.05) according to two-tailed Student’s t-test.