FIGURE 3.

NDUFA4L2 protected OS cells by repressing ROS production. 143b, U2OS, and HOS cells were treated with NAC post-transfection with si-NC, si-NDUFA4L2-1, or si-NDUFA4L2-2 in hypoxic environments. (A) Western blotting facilitated determinations of protein expression of Beclin-1, P62, LC3, Bax, Bcl-2, Cleaved-caspase3, PARP, and GAPDH in 143b, U2OS, and HOS cells. (B,D) The colonizing ability of si-NDUFA4L2-transfected 143b and U2OS cells post-treatment with NAC was determined by using colony formation assays. (C,E) Invasion ability of si-NDUFA4L2-transfected 143b and HOS cells post-treatment with NAC was determined by using transwell assays. HY: hypoxic environment. nsp ≥ 0.05, and # < 0.001 were defined as measures that indicated significant differences among treatment groups. All experiments were performed in triplicate.