Fig. 3.

Phylogeny, haplotype and structure among Histoplasma capsulatum genotypes. A. Phylogenetic relationships, as inferred from neighbor joining analysis of the ADP-ribosylation factor sequences (arf; n = 211 OTU), covering the main haplotypes of H. capsulatum. Numbers above the tree branches are the bootstrap values for NJ, ML and MP methods. The branches with bootstrap support higher than 70 % are indicated in bold. B. Median-joining haplotype network of H. capsulatum isolates, covering all the arf haplotypes found in this study. The size of the circumference is proportional to the haplotype frequency. The haplotypes are color coded according to the genetic group to which they were assigned. Mutational steps are represented by white dots. The black dots (median vectors) represent unsampled or extinct haplotypes in the population. C. Distribution patterns of H. capsulatum arf sequences used in this study. Note that clade naming follows the original appearance of the isolates in the literature (Kasuga et al., 2003, Taylor et al., 2005, Muniz Mde et al., 2010). NJ, neighbor joining; ML, maximum likelihood; MP, maximum parsimony. Further information about isolate source and GenBank accession number can be found in Table S1.