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. 2020 Nov 20;11:563402. doi: 10.3389/fimmu.2020.563402

Figure 3.

Figure 3

Effects of Immune Education on CLP-induced organ injury and CLP survival. C57Bl/6 mice were treated with anti-CD3ϵ (Educated – circles) or isotype control (Cont – squares) and were subjected to CLP 35 days later. Mice were sacrificed 24 h post-CLP, serum was obtained and RNA was extracted from liver. In a separate cohort, five-day survival from CLP was determined. Data as mean ± SEM, *p < 0.05 for all studies by two-way Student’s T test. Figures representative of at least two independent experiments. (A) Serum alanine aminotransferase (ALT) levels (N = 4–5/group). (B) Serum total bilirubin concentration (N = 9–10/group). (C) Relative hepatic abundance of mRNA encoding Sodium/Bile Acid Cotransporter (SCL10a1a). ΔΔCT values of each sample normalized to GAPDH to account for loading, mean value for abundance of control specimen arbitrarily set at unity. N= 5–6/group. (D) Relative hepatic abundance of mRNA encoding Organic Anion Transporter (SCLO1a1). ΔΔCT values of each sample normalized to GAPDH to account for loading, mean value for abundance of control specimen arbitrarily set at unity. N= 5–6/group. (E) Five-day Survival following CLP in educated (circles) and control (squares) mice. N = 28–29/group. p = 0.027 by Spearman’s Log-rank test.