Figure 12.

Analysis of P2RY12 expression in microglial clusters after FNA. (A–O) Representative double-immunolabeled images combining TREM2 (green) and P2RY12 (red) in the ipsilateral NL FN (A–C) and at 14 (D–I) and 21 dpI (J–O). Note that clusters with high levels of P2RY12 (arrows) can present both high and low levels of TREM2. In the same way, clusters with low levels of P2RY12 (arrowheads) show high and low levels of TREM2. No differences were detected between all the time-points in terms of P2RY12 staining. Scale bar (A–O) = 10 μm.