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. 2020 Nov 20;14:567404. doi: 10.3389/fncel.2020.567404

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Copyright © 2020 Manich, Gómez-López, Almolda, Villacampa, Recasens, Shrivastava, González and Castellano.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Analysis of the expression of TREM2 ligand Galectin-3 after PPT. (A–F) Representative double immunofluorescence images combining Galectin-3 (red) and TREM2 (green) at 2 dpI (A–C) and 3 dpI (D–F) after PPT. Occasional microglial cells showing TREM2 expression in their ramifications contained Galectin-3 (white arrowheads) as perinuclear staining in both time-points. Note that at 3 dpI some microglial cells also presented Galectin-3 in their ramifications, indicating an increase of Galectin-3 compared to 2 dpI. Scale bar (A–F) = 10 μm.