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. 2020 Nov 20;7:592550. doi: 10.3389/fcvm.2020.592550

Figure 2.

Figure 2

Effect of Forced expression or knockdown of FTO on proliferation and migration of VSMCs. (A) Human VSMCs were infected with Lentivirus encoding FTO (pLV-FTO) or pLV-GFP for 48 h, and then western blot detected the expressions of FTO and Klf5. (B) MTT assay for cell proliferation in pLV-FTO-infected VSMCs. *P < 0.05, **P < 0.01 vs. pLV-GFP at the corresponding time point. (C) A scratch assay for the pLV-GFP- or pLV-FTO-infected VSMCs at 24 h later. Right: statistic of migrating cells in the scratch gap. n = 3. *P < 0.05 vs. pLV-GFP. (D) VSMCs were transfected with siRNA against FTO (si-FTO) or non-specific siRNA (si-Con) for 24 h, and then the expressions of FTO and Klf5 were examined by western blot. (E) MTT assay for effects of FTO knockdown on cell proliferation. *P < 0.05 vs. si-Con at the corresponding time point. (F) Scratch assay for the si-Con or si-FTO-transfected VSMCs at 24 h later. Right: statistic of migrating cells in the scratch gap. The data present mean ± SEM from three independent experiments. *P < 0.05 vs. si-Con.