Figure 2.
The antibody-immobilized chip generated a sub-optimal calibration curve but displayed a better α-hemolysin selectivity. The calibration curve for the antibody-immobilized chip was established in the same way as for the MIP (A). Here, a double response (RU) was not seen as a result of doubling in α-hemolysin concentrations which effectively decreased both the dynamic range and the degree of fit (R2 = 0.829). Contrarily, the antibody-immobilized chip proved to be highly selective for α-hemolysin, with little or no response recorded for all potential blood-sample contaminants (B). Values are presented as the mean of triplicates with standard deviations. A one-way ANOVA analysis was used to evaluate selectivity results. ****P < 0.0001; ND, Non-Detectable.