Table 1.
With the exception of albumin, the antibody-immobilized chip showed greater selectivity coefficients for all potential cross-reactants.
| Analyte | Response (RU) MIP chip | K(MIP chip) | Response (RU) NIP chip | K(NIP chip) | Response (RU) Immobilized chip | K(Immobilized chip) |
|---|---|---|---|---|---|---|
| Alpha hemolysin | 733.0 | – | 67.82 | – | 9.4 | – |
| Keratin | 246.6 | 2.97 | 965.4 | 0.07 | 0.3267 | 28.77 |
| Collagen | 143.5 | 5.10 | 222.7 | 0.30 | 0.2833 | 33.18 |
| Cholera Toxin | 71.44 | 10.26 | 420.2 | 0.16 | 0.9 | 10.44 |
| Fibrinogen | 91.21 | 8.04 | 69.1 | 0.98 | ND | ND |
| IgG | 59.49 | 12.32 | 88.44 | 0.77 | ND | ND |
| Albumin | 4.723 | 155.20 | 61.29 | 1.10 | 0.08 | 117.50 |
No interaction could be detected between the immobilized chip and fibrinogen or IgG. Interactions with potential skin-swab contaminants were also lower (generating a higher selectivity coefficient). Only albumin had a percentually lower binding to the MIP. Selectivity coefficients (K) were calculated using: Response (α-hemolysin)/Response (cross-reactant).