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Cytotoxicity of TPL@nano-gel against MDA-MB-231 and MCF-7 human breast cancer cells, compared with free TPL. MDA-MB-231 and MCF-7 cells were treated with various concentrations of free TPL and TPL@nano-gel (0–160 nmol/L) for 24, 48, 72 and 96 h, respectively. Cell viability was assessed by MTT assay. IC50 values of cell viability in MDA-MB-231 and MCF-7 cells with free TPL and TPL@nano-gel treatment were calculated and analyzed by software GraphPad Prism 5.0 (mean ± SD, n = 3).
