Fig. 2.

ACN induces PS exposure. (A) Representative histogram of Annexin-V-FITC fluorescence of control cells. (B) Representative histogram of Annexin-V-FITC fluorescence of cells treated with 30 µM ACN. (C) Arithmetic means ± SEM (n = 9) of ACN-induced dose-dependent PS exposure. Cells were treated with the vehicle control or with ACN at 10–30 µM for 24 h at 37 °C and PS exposure was detected by Annexin-V-FITC staining. ***(P < 0.001) indicates significant difference from control (ANOVA).