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. 2020 Sep 8;27(12):3376–3384. doi: 10.1016/j.sjbs.2020.09.005

Fig. 4.

Fig. 4

ACN elevates cytosolic calcium. (A) Representative histogram of Fluo4 fluorescence of control cells. (B) Representative histogram of Fluo4 fluorescence of cells treated with 30 µM ACN. (C) Arithmetic means ± SEM (n = 9) of ACN-induced dose-dependent calcium overload. (D) Arithmetic means ± SEM (n = 9) of the percentage of cells with increased Fluo4 fluorescence. Cells were treated with the vehicle control or with ACN at 10–30 µM for 24 h at 37 °C and cytosolic calcium was measured by Fluo4/AM staining. **(P < 0.01) and ***(P < 0.001) indicate significant difference from control (ANOVA).