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ACN increases ROS levels. (A) Representative histogram of DCF fluorescence of control cells. (B) Representative histogram of DCF fluorescence of cells treated with 30 µM ACN. (C) Arithmetic means ± SEM (n = 9) of ACN-induced dose-dependent ROS increase. Cells were treated with the vehicle control or with ACN at 10–30 µM for 24 h at 37 °C and ROS were quantified by H2DCFDA staining. *(P < 0.05) indicates significant difference from control (ANOVA).
