Figure 3.

Halofuginone indirectly affected the frequency of infiltrating myeloid cells in gingival tissues. WT mice were pretreated with 500 μg/mL Halofuginone. 24 h later, a chronic periodontitis model was induced in these mice as indicated. (a) The frequency of indicated cell-types in gingival tissues was analyzed by flow cytometry 3 weeks after the treatment (n = 4). (b) BMs-derived osteoclast precursors were treated with various concentrations of Halofuginone for 24 h, and cell viability was determined by MTT assay. (c) BM were cultured in M-CSF (25 ng/mL) and RANKL (50 ng/mL) for 3 days. The differentiation of osteoclast was analyzed by TRAP staining.

Data are presented as mean ± SEM values and representative of at least three independent experiments. Statistical analyzes represent variations in experimental replicates.

*P < 0.05, **P < 0.01.