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. 2020 Dec 2;11:2041731420975569. doi: 10.1177/2041731420975569

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© The Author(s) 2020

This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access page (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Figure 4. — Effects of hDPSC-EVs on the MAPK pathway in hADSCs. (a) ALP staining of hADSCs after treatment with hDPSC-EVs and/or inhibitors. (b) Quantitative detection of ALP activity of hADSCs treated with hDPSCEVs and/or inhibitors. (c–e) Protein expression of p-P38/P38, p-ERK 1/2 1/2/ERK 1/2, and p-JNK/JNK, determined by western blotting. (f–h) The ratios of p-P38/P38, p-ERK 1/2 1/2/p-ERK 1/2, and p-JNK/JNK intensity. SB: SB203580, inhibitor of P38; PD: PD98059, inhibitor of ERK 1/2; and SP: SP600125, inhibitor of JNK. Data are presented as the mean ± standard deviation of the mean (n = 3), ** represents p < 0.01.