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. 2020 Nov 1;13(11):2806–2819.

Figure 5.

Figure 5

CDK6 is a direct target of miR-770-5p. A. Starbase software was applied to analyze the relationship between miR-770-5p and CDK6. B, C. The binding sites of CDK6 in miR-770-5p were verified by dual-luciferase reporter assay in AGS and MKN45 cells. D, E. Levels of miR-770-5p or CDK6 were assessed in AGS and MKN4 transfected with miR-NC, miR-770-5p, anti-miR-NC or anti-miR-770-5p. F. CDK6 protein level in transfected AGS and MKN4 cells was detected by western blot. G, H. Both mRNA level and protein level of CDK6 were examined in GC tissues and adjacent normal tissues. I. Pearson’s correlation analysis was conducted to analyze the association between miR-770-5p and CDK6 in GC tissues. J, K. CDK6 level in GES-1, AGS, and MKN45 cells was tested by RT-qPCR assay and western blot. *P < 0.05.