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. 2020 Nov 1;10(11):3705–3720.

Figure 4.

Figure 4

GOLM1 upregulates PD-L1 expression in HCC cell lines. A and B. Expression levels of GOLM1 in different HCC cell lines: HCC-LM3, Huh7, Hep3B and Bel-7402. C and D. Expression levels of PD-L1 in different HCC cell lines: HCC-LM3, Huh7, Hep3B and Bel-7402. Protein expression was detected by Western blotting. mRNA expression was detected by RT-qPCR. E and F. Western blot and RT-qPCR analysis of PD-L1 levels in GOLM1-knockdown Huh7 cells. Quantitative analysis of GOLM1 and PD-L1 expression after GOLM1 was knockdown in Huh7 cells through Image J intensity measurement. G and H. Western blot and RT-qPCR analysis of PD-L1 levels in GOLM1-overexpressed Hep3B cells. Quantitative analysis of GOLM1 and PD-L1 expression after GOLM1 was overexpressed in Hep3B cells through Image J intensity measurement. **P < 0.01, ***P < 0.001, ****P < 0.0001.