Figure 3.

ERRα promotes PC cell proliferation and tumor growth. A-C. CCK-8, EdU and colony formation assays were applied to determine the proliferation of PaTu8988 and PANC1 cells after ERRα depletion, and that of Mia PaCa-2 cells after ERRα overexpression. D. The mRNA and protein expression level of ERRα in PaTu8988 cells transfected with LV-ERRα, LV-shERRα or their empty vectors. E. Compared with empty vector groups, overexpression of ERRα promoted while knockdown of ERRα suppressed the tumor growth in nude mice. F. Tumor weight was measured after mice sacrificed and tumor resection; Tumor growth curves were plotted. *P<0.05, **P<0.01, ***P<0.001.