Figure 5.

Knockdown of ERRα induces cell apoptosis and G0/G1 arrest in PC cells. A. Flow cytometric analysis of apoptosis in PaTu8988 and PANC1 cell after ERRα knockdown. B. Western blot analysis of apoptosis related markers. C. After silencing of ERRα, flow cytometry showed that cell cycles of PaTu8988 and PANC1 cells were arrested at G0/G1 phase. D. Cell cycle related proteins were analyzed by western blotting. E. The expression levels of cleaved-PARP, cleaved-caspase 3, cyclin D1 and p27 Kip1 were detected by IF assay. *P<0.05, **P<0.01, ***P<0.001.