Table 1.

Virulence factor (VF)-encoding genes detected in the 197 canine E. coli isolates. Relationship with phylogenetic groups.

VF-Encoding Gene	Number of Isolates (%)
	Total (n = 197)	A (n = 32)	B1 (n = 26)	B2 (n = 84)	C (n = 10)	D (n = 6)	E (n = 14)	F (n = 18)	Clade V (n = 1)	NT 1 (n = 6)
Adhesins
fimH	192 (97.5)	29	24	84	10	6	14	18	1	6
fimAvMT78	20 (10.2)	12	1	1	0	0	2	3	0	1
papAH	62 (31.5)	2	3	54	0	1	1	1	0	0
papC	62 (31.5)	2	3	54	0	1	1	1	0	0
sfa/focDE	66 (33.5)	0	2	63	0	0	0	1	0	0
afa/draBC	1 (0.5)	0	0	0	0	1	0	0	0	0
yfcV	96 (48.7)	4	0	80	0	0	0	11	0	1
Toxins
sat	3 (1.5)	0	0	2	0	0	0	1	0	0
cnf1	54 (27.4)	0	0	53	0	0	0	1	0	0
hlyA	56 (28.4)	0	2	54	0	0	0	0	0	0
cdtB	8 (4.1)	0	0	6	0	0	0	2	0	0
tsh	21 (10.7)	2	2	0	8	0	2	6	0	1
vat	79 (40.1)	1	0	76	0	0	0	2	0	0
Iron uptake
iutA	33 (16.8)	3	3	7	8	1	2	9	0	0
iroN	93 (47.2)	3	6	66	8	0	2	8	0	0
fyuA	106 (53.8)	7	2	77	8	5	1	5	0	1
chuA	122 (61.9)	0	0	84	0	6	14	18	0	0
Capsule
kpsM II	64 (32.5)	1	0	41	0	4	4	10	1	3
neuC-K1	12 (6.1)	0	0	10	0	0	0	2	0	0
kpsM II-K2	7 (3.6)	0	0	2	0	3	0	1	0	1
kpsM II-K5	45 (22.8)	1	0	29	0	1	4	7	1	2
kpsM III	6 (3.0)	1	0	2	0	2	1	0	0	0
Miscellaneous
cvaC	21 (10.7)	1	4	2	8	0	0	6	0	0
iss	31 (15.7)	2	5	6	8	0	2	8	0	0
traT	59 (29.9)	2	12	17	8	2	7	10	0	1
ibeA	54 (27.4)	0	0	49	0	0	0	3	0	2
malX	93 (47.2)	0	5	79	0	0	0	7	0	2
usp	86 (43.7)	1	3	77	0	1	1	2	0	1
ExPEC status	74 (37.6)	2	2	61	0	1	1	7	0	0
UPEC status	82 (41.6)	1	0	77	0	0	0	4	0	0
Range of VFs	0 to 18	0 to 10	1 to 10	2 to 17	1 to 8	5 to 7	2 to 7	3 to 18	3	1 to 9
Mean of VFs	7.87	2.31	2.96	12.79	6.60	5.67	4.14	7.94	3.00	3.67

¹ Using the revised protocol developed by Clermont et al. [43] six isolates were not typeable (NT). These six isolates belonged to phylogroup A using the first protocol developed by Clermont et al. [43] that classifies isolates into only four phylogenetic groups (A, B1, B2, D).