Skip to main content
. 2020 Nov 25;76(Pt 12):604–608. doi: 10.1107/S2053230X20015204

Table 2. Crystallization of hisZC3H41ΔN331 .

Method Vapor diffusion
Plate type 24-well VDX hanging-drop plate, Hampton Research
Temperature (K) 289
Protein concentration (mg ml−1) 14
Buffer composition of protein solution 30 mM HEPES pH 7.5, 200 mM NaCl, 10% glycerol, 5 mM β-mercaptoethanol
Composition of reservoir solution 16%(v/v) PPGBA 2000, 0.2 M imidazole pH 7
Volume and ratio of drop 2 µl (1:1)
Volume of reservoir (µl) 400