Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
| Method | Sitting-drop |
| Plate type | 96-well sitting drop |
| Temperature (K) | 280 |
| Protein concentration (mg ml−1) | 7 |
| Buffer composition of protein solution | 50 mM Tris pH 8.0, 10 mM NaCl |
| Composition of reservoir solution | 0.2 M MES pH 6, 0.2 M CaCl2, 20% PEG 6000 |
| Volume and ratio of drop | 200 nl:200 nl |
| Volume of reservoir (µl) | 50 |
