Table 2. Crystallization conditions for diffracting crystals.
| Method | Hanging-drop vapour diffusion |
| Temperature (K) | 293 |
| Protein concentration (mg ml−1) | 7.7 |
| Buffer composition of protein solution | 50 mM HEPES pH 6.5, 5 mM DTT, 100 mM or 50 mM NaCl |
| Composition of reservoir solution | 0.08 M sodium cacodylate pH 6.5, 0.16 M magnesium acetate, 16%(v/v) polyethylene glycol 8000, 20%(v/v) glycerol |
| Volume and ratio of drop | 2 µl, 1:1 protein:reservoir solution |
| Volume of reservoir (µl) | 150 |