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. 2020 Nov 25;76(Pt 12):590–596. doi: 10.1107/S2053230X20014880

Table 2. Crystallization conditions for diffracting crystals.

Method Hanging-drop vapour diffusion
Temperature (K) 293
Protein concentration (mg ml−1) 7.7
Buffer composition of protein solution 50 mM HEPES pH 6.5, 5 mM DTT, 100 mM or 50 mM NaCl
Composition of reservoir solution 0.08 M sodium cacodylate pH 6.5, 0.16 M magnesium acetate, 16%(v/v) polyethylene glycol 8000, 20%(v/v) glycerol
Volume and ratio of drop 2 µl, 1:1 protein:reservoir solution
Volume of reservoir (µl) 150