Table 2. Molar Mass Recoveries for EC and Its Colonic Metabolites (%) during 24 Hours of Anaerobic Incubation with a Pooled Fecal Sample from 14 Donors.
| chemical | 0 (h) | 1 (h) | 2 (h) | 3 (h) | 4 (h) | 5 (h) | 6 (h) | 24 (h) |
|---|---|---|---|---|---|---|---|---|
| ECb | 88.54 ± 11.51 | 24.92 ± 5.21 | 5.86 ± 0.68 | 2.74 ± 0.86 | 0.04 ± 0.03 | 0.01 ± 0.01 | 0.03 ± 0.03 | n.d.d |
| 3,4-diHPP-2-olc | n.d. | 71.92 ± 5.61 | 70.13 ± 13.47 | 23.74 ± 10.42 | n.d. | n.d. | n.d. | n.d. |
| 3-HPP-2-olc | n.d. | 2.72 ± 0.27 | 10.86 ± 0.84 | 16.32 ± 3.89 | 2.22 ± 1.93 | n.d. | n.d. | n.d. |
| 4H-diHPVAc | n.d. | n.d. | 0.79 ± 0.10 | 2.64 ± 0.04 | 4.39 ± 0.42 | 6.20 ± 0.14 | 7.68 ± 0.39 | n.d. |
| 3,4-diHPVb | n.d. | 4.48 ± 1.08 | 21.78 ± 2.19 | 46.19 ± 6.37 | 49.19 ± 6.48 | 47.48 ± 6.37 | 45.01 ± 6.25 | 0.04 ± 0.08 |
| 3-HPVb | n.d. | n.d. | 0.75 ± 0.12 | 11.03 ± 2.15 | 24.68 ± 1.62 | 24.51 ± 1.80 | 23.76 ± 2.75 | 0.17 ± 0.14 |
| 3,4-diHPVAb | n.d. | n.d. | 0.14 ± 0.07 | 0.39 ± 0.10 | 0.68 ± 0.16 | 1.08 ± 0.29 | 1.69 ± 0.40 | n.d. |
| 3-HPVAb | n.d. | 0.02 ± 0.02 | 0.06 ± 0.06 | 0.09 ± 0.09 | 0.36 ± 0.18 | 0.79 ± 0.34 | 1.73 ± 0.71 | 86.14 ± 15.61 |
| 3-HPPAb | n.d. | 0.66 ± 0.22 | 2.34 ± 0.95 | 2.94 ± 0.75 | 3.14 ± 0.65 | 3.16 ± 0.40 | 2.95 ± 0.62 | n.d. |
| 3-HPAAb | n.d. | 0.03 ± 0.23 | 0.16 ± 0.18 | n.d. | n.d. | n.d. | n.d. | n.d. |
| total | 88.54 | 104.75 | 112.88 | 106.08 | 84.70 | 83.23 | 82.85 | 86.35 |
a
Results are shown as mean ± SD from three independent incubations.
b
Qualitatively and quantitatively detected by LC-TQ-MS.
c
Qualitatively and quantitatively detected by LC-TOF-MS.
d
Not detected.