Figure S2.

cen mRNA granule formation requires the centrosome scaffold. (A) Image shows immunofluorescence for Cnn (green) and cen smFISH (magenta) in an NC 12 cnn^B4 embryo. Boxed region is enlarged in inset. Note the absence of large pericentrosomal cen mRNA granules. (B) Immunoblots show Cen protein content in 0–2-h (up to NC 14) WT and cnn^B4 lysates. Actin is used as a loading control. (B′) Each dot represents the levels of Cen normalized to the mean relative expression of the actin load control. n.s., not significant (P = 0.672) by unpaired t test from n = 3 independent biological replicates, with n = 2 technical replicates run on the same gel. (C–G′) Images show interphase NC 12 embryos stained for Cnn (magenta) and antibodies for the indicated RNA-binding proteins (RNA-binder, green): Egl (C), Orb2 (D), me31B (E), Pum (F), and FMRP (G). (G′) Inset from G; arrowheads, FMRP overlapping with Cnn. Scale bars: 10 µm; 2 µm (insets).