Figure S3.

VAIL is induced by transfection of poly(dA:dT) independently of STING. (a) WT and FIP200 KO HeLa cells were transfected with 4 μg/ml poly(dA:dT) (dsDNA) and Lipofectamine 2000 at (1:1.5 μg DNA: μl Lipofectamine) alone and with BafA1 (100 nM) for 4 h. All the following experiments were treated the same unless specified. (b) WT and ATG16L KO HeLa cells with stable expression of GFP-STING and BFP-ATG16L-α, BFP-ATG16L-β, or ATG16L-ΔWD40 were transfected with dsDNA. (c) WT HeLa cells and WT HeLa cells with stable expression of BFP-SopF were transfected with dsDNA. (d) WT, STING KO, and STING KO HeLa cells stably expressing GFP-STING were transfected with dsDNA.