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. 2020 Nov 24;23(1):87. doi: 10.3892/mmr.2020.11728

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Copyright: © Wang et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 2. — Fractionated irradiation enriches CSC characteristics in human bladder cancer cells. (A) Representative images (scale bar, 200 µm) and (B) quantification of tumorspheres formed by 5637/5637R and T24/T24R cells. The number of tumorspheres was counted in five independent fields/well after 1 week of cultivation. (C) Representative images and (D) quantification of soft agar colony formation analysis of 5637/5637R and T24/T24R cells. The data are presented as the mean ± SEM of triplicate experiments. (E) Results of the tumor formation assays of saline control, 5637, 5637R, T24 and T24R groups (n=3). R, radio-resistant cells; CSC, cancer stem cells.