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. 2020 Nov 24;23(1):87. doi: 10.3892/mmr.2020.11728

Figure 4.

Figure 4.

Depletion of STAT3 abrogates the self-renewal and tumorigenicity of human BCa cells. (A) Kaplan-Meier curves comparing the RFS and OS between patients with BCa expressing high or low levels of STAT3, a log-rank test was performed (left, RFS; right, OS). (B) Western blot analysis of STAT3, GAPDH served as a loading control. (C) Quantification of migration analysis of siCtrl and siSTAT3 BCa cells. (D) Quantification of invasion analysis of siCtrl and siSTAT3 BCa cells. (E) Quantification of tumorspheres formed by siCtrl and siSTAT3 BCa cells. (F) Quantification of soft agar colony formation analysis of siCtrl and siSTAT3 BCa cells. The data are presented as the mean ± SEM of triplicate experiments. (G) Results of the tumor formation assays of siCtrl and siSTAT3 BCa cells (n=3). STAT3, signal transduction and transcription activator 3; BCa, bladder cancer; RFS, relapse-free survival; OS, overall survival; si-, small interfering RNA; Ctrl, control; HR, hazard ratio; R, radio-resistant cells.