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Flow cytometric and CCK-8 assays were performed to confirm that Klotho protein and Nec-1 attenuate the Ang II induced cardiotoxicity in H9c2 cells. (A) The flow cytometric chart and (B) corresponding quantified statistical data. (C) Results of Cell Counting Kit-8 assays. Data are expressed as the mean ± SD. n=6. *P<0.05, vs. the control NC. #P<0.05, vs. Ang II. CCK-8, Cell Counting Kit-8; Nec-1, necrostatin-1; Ang II, angiotensin II; NC, negative control.
