Figure 3.

Effect of Klotho protein treatment on ROS of H9c2 after Ang II treatment. Intracellular ROS generation was measured by fluorescence imaging using DCFH-DA. Representative microscopy images of DCFH-DA fluorescence in Ang II-induced H9c2 cells after 24 h revealed that H9c2 in the model group, treated with Ang II produced a significantly increased level of ROS compared with the untreated group (control). However, the ROS content of the Klotho protein- and Nec-1-treated group gradually decreased. Results are expressed as the mean ± standard error. n=5. *P<0.05 vs. NC. ^#P<0.05 vs. Ang II. ROS, reactive oxygen species; Ang II, angiotensin II; DCFH-DA, 2,7-dichlorodihydrofluorescein diacetate; Nec-1, necrostatin-1; MFI, mean fluorescence intensity.