Figure 6.

Effect of TLR4 and NF-κB inhibitors on RIP3 and MLKL expression (A) TLR4 inhibitor TAK-242 suppressed p-NF-κB p65, RIP3 and MLKL protein expression upregulation, stimulated by Ang II in H9c2 cells. (B) NF-κB p65 inhibitor PDTC suppressed RIP3 and MLKL protein expression upregulation, stimulated by Ang II in H9c2 cells. Data are expressed as the mean ± SD. n=3. *P<0.05 vs. NC group, ^#P<0.05 vs. the Ang II-treated group. TLR4, toll-like receptor 4; NF-κB, nuclear factor-κB; RIP3, receptor-interacting protein kinase 3; MLKL, mixed-lineage kinase domain-like protein; p-, phosphorylated; Ang II, angiotensin II; PDTC, pyrrolidine dithiocarbamate; NC, negative control.