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. 2020 Nov 16;23(1):51. doi: 10.3892/mmr.2020.11689

Figure 5.

Figure 5.

miR-21-5p overexpression may protect melanocytes via regulating T effector/regulatory T cell balance through STAT3. (A) Apoptosis rates of melanocytes in the control, MC+NC, M+NC, MC+STAT3 and M+STAT3 groups were measured via flow cytometry. Protein levels of (B) IL-17A and (C) IL-22 in the lower chamber of Transwell inserts after co-culture of melanocytes and Th17-polarized CD4+ T cells in the control, MC+NC, M+NC, MC+STAT3 and M+STAT3 groups was measured via enzyme-linked immunosorbent assay. (D) Tyrosinase activity and (E) relative melanin content in melanocytes in the control, MC+NC, M+NC, MC+STAT3 and M+STAT3 groups were measured using a microplate reader. *P<0.05, **P<0.01 and ***P<0.001 vs. MC+NC; ^P<0.05 and ^^P<0.01 vs. MC+STAT3; ##P<0.01 and ###P<0.001 vs. M+NC. Th, T helper; IL, interleukin; miR, microRNA; M, miR-21-5p mimic; MC, mimic control; NC, negative control.