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. 2020 Nov 23;23(1):85. doi: 10.3892/mmr.2020.11724

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Copyright: © Wang et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 4. — Downregulation of SNHG4 expression decreases the effects of miR-204-5p inhibition on the proliferation and apoptosis of gastric cancer cells. Relative SNHG4 expression levels in siNC+IC, siNC+I, siSNHG4+IC and siSNHG4+I groups in (A) AGS or (B) HGC-27 cells. OD in each group in (C) AGS or (D) HGC-27 cells. Relative colony number in each group in (E) AGS or (F) HGC-27 cells. Protein expression levels of PCNA and cyclin D1 in (G) AGS or (H) HGC-27 cells in siNC+IC, siNC+I, siSNHG4+IC and siSNHG4+I groups. Apoptosis rate of (I) AGS or (J) HGC-27 cells in each group. Bars indicate the mean ± standard deviation. *P<0.05, **P<0.01 and ***P<0.001 vs. siNC+IC; ^{^}P<0.05, ^{^^}P<0.01 and ^{^^^}P<0.001 vs. siNC+I; ^#P<0.05, ^##P<0.01 and ^###P<0.001 vs. siSNHG4+IC. SNHG4, small nucleolar RNA host gene 4; miR, microRNA; si, short interfering; NC, negative control; IC, inhibitor control; I, inhibitor; OD, optical density; PCNA, proliferating cell nuclear antigen; lncRNA, long non-coding RNA.