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. 2020 Nov 19;23(1):67. doi: 10.3892/mmr.2020.11706

Figure 2.

Figure 2.

Positive feedback loop between p38 MAPK and necroptosis pathways in HUVECs. (A) Expression levels of RIP3 were semi-quantified by western blotting analysis. HUVECs were treated with 40 mM HG for 24 h with or without pretreatment with 3 µM SB203580 for 1 h. (B) Expression levels of p-p38/p38 were analyzed using western blotting. HUVECs were treated with 40 mM HG for 24 h with or without pretreatment with 100 µM Nec-1 for 24 h. (C) Transfection efficiency of RIP3-siRNA in HUVECs was determined using western blotting. (D) Expression levels of p-p38/p38 were determined using western blotting with or without RIP3-siRNA transfection. Data are presented as the mean ± SEM (n=3). *P<0.05 vs. control group; #P<0.05, ##P<0.01 vs. HG group; +P<0.05 vs. RIP3-siRNA group. RIP3, receptor-interacting protein 3; p-, phosphorylated; HG, high glucose; Nec-1, necrostatin-1; HUVECs, human umbilical vein endothelial cells; siRNA, small interfering RNA; NC, negative control.