Figure 4.

Role of necroptosis and p38 MAPK inhibition in the protective effects of NaHS against HG-induced ROS generation in HUVECs. After the HUVECs were treated with the indicated treatments, intracellular ROS generation was measured using 2′7′-dichlorodihydrofluoresein diacetate staining followed by fluorescence microscopy. Scale bar, 50 µm. Data are presented as the mean ± SEM (n=5). **P<0.01 vs. control group; ^##P<0.01 vs. HG group. NaHS, sodium hydrosulfide; HG, high glucose; HUVECs, human umbilical vein endothelial cells; RIP3, receptor-interacting protein 3; siRNA, small interfering RNA; ROS, reactive oxygen species; MFI, mean fluorescence intensity.