Figure 5.

Role of necroptosis and p38 MAPK inhibition on the protective effects of NaHS against the HG-induced loss of MMP in HUVECs. After the HUVECs were treated with the indicated treatments, the MMP was analyzed using the fluorescent dye, Rhodamine 123, followed by fluorescence microscopy. Scale bar, 50 µm. Data are presented as the mean ± SEM (n=5). **P<0.01 vs. control group; ^#P<0.05, ^##P<0.01 vs. HG group. NaHS, sodium hydrosulfide; HG, high glucose; HUVECs, human umbilical vein endothelial cells; RIP3, receptor-interacting protein 3; siRNA, small interfering RNA; MMP, mitochondrial membrane potential; MFI, mean fluorescence intensity.