Table 2.
Analysis of different linkers using ProtParam server
| Linker | Sequence | II of the fusion protein | Aliphatic Index | Gravy | |
|---|---|---|---|---|---|
| Flexible linkers | LF1 | (G8) GGGGGGGG | 35.08 | 74.92 | −0.455 |
| LF2 | (G4S) GGGGS | 32.45 | 76.15 | −0.459 | |
| LF3 | (G4S)2 GGGGSGGGGS | 33.82 | 74.12 | −0.459 | |
| LF4 | GSAGSAAGSGEF | 29.26 | 74.92 | −0.425 | |
| LF5 | KESGSVSSEQLAQFRSLD | 34.65 | 77.08 | −0.487 | |
| LF6 | EGKSSGSGSESKST | 32.26 | 72.57 | −0.537 | |
| Rigid Linkers | LR1 | EAAAK | 31.55 | 77.80 | −0.456 |
| LR2 | A (EAAAK)2AA | 30.06 | 77.68 | −0.419 | |
| LR3 | (XP)n = APAPAP | 35.88 | 77.38 | −0.440 | |
| LR4 | (XP)n = EPEPEP | 35.63 | 75.74 | .527 | |
| LR5 | (XP)n:KPKPKP | 28.63 | 75.74 | −0.533 | |
| LR6 | (XP)n: GPGP | 30.32 | 76.57 | −0.470 |