Fig. 2.

Knockdown of EZH2 increased the sensitivity to gefitinib in EGFR wild-type A549 and H1299 cells. a EZH2 protein levels were assessed by western blotting. b Quantitative PCR analysis of relative EZH2 mRNA expression level normalized to GAPDH expression level. c-d Cell proliferation of A549 and H1299 cells transfected with siNC or siEZH2 was measured using the CCK-8 assay. Data are representative of three independent experiments.e The IC50 of GSK343,DZNep and gefitinib were analysis by CCK-8 assay. All results are presented as mean ± standard deviation (SD) of triplicate experiments. * P < 0.05, ** P < 0.01 vs. the gefitinib alone-treated group