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. 2020 Dec 4;6:39. doi: 10.1186/s40813-020-00177-9

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — Pen mean lung lesion scores for tylvalosin pigs (TVN group) and untreated pigs (CTL group) following experimental inoculation with M. hyopneumoniae alone (Mhyop-only) or in a sequential combination with P. multocida (Mhyop + Pm). Box plots represents the arithmetic mean of each pen. In Mhyop-only, there were 6 pigs in each pen, 17 pens for each treatment. Pigs were challenged endotracheally with lung homogenate containing 1 × 10⁵ ccu of M. hyopneumoniae in 10 ml on Day 0 and Day 1 and scored on Day 28. In Mhyop+Pm, there were 4 pigs in each pen, 11 pens for each treatment. Pigs were challenged on Day 0, Day 1 and Day 3 with broth media containing 1 × 10⁹ cfu of M. hyopneumoniae in 5 ml of Mhyop and on Day 14 with broth media containing 1 × 10¹⁰ cfu in 10 ml of P. multocida. Both challenges through intranasal route and scored on Day 28. P value from least square means derived from statistical models